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Post by DarJones on Mar 7, 2010 22:20:28 GMT -5
Alan, You are missing the point of breeding a population that is pure se. It requires inbreeding.
Take a single stalk of corn. Put a white paper bag over the ear just before it tassels. When it tassels, dust pollen onto its own silks. End result is that a stalk that is pure se/se will produce pure se kernels and can then be used to build a population of true breeding corn. The bad part about doing this is that you have to grow at least 200 stalks to get 50 that are pure se/se and each one has to be bagged and hand pollinated. When they are harvested, the result will be a very pure se/se corn.
If you are trying to develop a true beeding green corn, you should be using similar methods.
DarJones
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Post by Alan on Mar 7, 2010 22:47:52 GMT -5
Yes, it can theoretically be done that way, but from talking to many others who have worked with the SE gene and from using inbreeding myself I no that there is no one who has had success in breeding an SE population in this way.
For color it works, for plant type it works, for Homozygous SE it hasn't yet worked. My guess is that regardless your going to always end up with SU/SE or else we would already have OP SE lines but we don't and to my knowledge there aren't any, add to that color genetics and SE Homozygous traits together and it becomes even harder to suss out.
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Post by DarJones on Mar 9, 2010 8:10:48 GMT -5
The problem with breeding corn is that the pollen grain is composed of 2 separate nuclei. One of them fertilizes the endosperm and the other goes to the nucleus that will germinate and produce the next generation of corn. In a mixed population, it is perfectly normal to get a single pollen grain that contains 1 nucleus for white and another for red. If this fertilizes a basic white corn, the result can be a white kernel of corn but that will produce red corn when it is grown.
This is the same concept with the se gene. There are plenty of commercial se varieties available that are pure se. It does not matter that they are hybrids, what does matter is that to produce a pure se hybrid they had to cross two pure se lines. If the professional plant breeders can produce a pure se line, then so can you. It does not require rocket science to do it.
Alan, That nobody has done it so far in no way precludes that it can be done. It just means that nobody has done it the right way. Your answer shows that you don't understand the genetics and most likely that the people you have spoken to don't either.
Silverseeds, Do the due diligence. Selfing is the most viable way to do this. One generation of selfing followed by a generation of crossing between the offspring of several carefully selected selfed plants should do the job.
I have seed from Cherokee Squaw corn. This is a variety that produces a mix of white and purple kernels on cobs that can be either white or red. If I want to isolate a line that produces only white corn on white cobs, I would have to grow only white kernels from only white cobs to start the breeding line. Then I would self about 200 plants which should produce about 16 plants that breed true for white kernels and white cobs. In the next growing season, I would let the kernels saved from the 16 selected plants cross at random and it should produce white kernels on white cobs.
DarJones
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Post by spero on Mar 9, 2010 10:26:09 GMT -5
We are getting some pretty high powered discussion going and out here only somewhat tied to the triploid discussion. I propose to open a couple of new lines. first, Selfing Vs Mass population to create pure se corn. - JS
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Post by Joseph Lofthouse on Mar 10, 2010 0:34:19 GMT -5
The problem with breeding corn is that the pollen grain is composed of 2 separate nuclei. One of them fertilizes the endosperm and the other goes to the nucleus that will germinate and produce the next generation of corn. In a mixed population, it is perfectly normal to get a single pollen grain that contains 1 nucleus for white and another for red. DarJones As I currently understand Meiosis and Mitosis in corn pollen, both nuclei in the pollen grain contain the same haploid set of chromosomes. (Which explains why I erased my first two posts in the triploid thread. I didn't want my misunderstanding to be disseminated any further.) Regards, Joseph
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Post by Andre on Jul 8, 2013 11:24:15 GMT -5
This link is broken. Do you have another one ?
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