Usually embryo rescue is required due to endosperm failure. The embryo cannot grow without the nutrients that would normally be provided by the endosperm. So, I don't see how you could pull it off in soil. Normally, the embryo is cultured on a nutrient rich medium that substitutes for the endosperm.
Growing where temperate rainforest meets the sea (WA coast): Jan avg low temp ~34*F, Aug avg high temp ~69*F, ~111 annual inches of rain, but only about 15 inches May-Sep, salt air, lots of wind.
Post by philagardener on Dec 26, 2017 18:28:08 GMT -5
A complication may be that all your embryos are literally in one basket - staging when to harvest the developing fruit may be a bit tricky at first, but days after pollination should be a way to standardize. Good luck and keep us posted!
Days after pollination may not be a constant, due to temperature etc. But it is often true that the embryo is good for several days. Generally, one wants to let the embryo grow naturally as long as possible, then rescue it. But the embryo might have been recuable over a few days. Depends on the cross.
billw. What you said above is generally true. One exxception I know of is Zea x Tripsicum hybrids. Sometimes the embro grows well enough, but the endosperm starts breaking down. By the time the embryo is mature enough, the endosperm is gone. By sdetting the embryo upright in sterile soil and keeping it from drying out, the light on the upper embryo can feed what is pretty much a tiny plant, though it is indeed an embryo. I learned that studying the history of Zea x Tripsicum. This was the first way to do it, other than just make the cross thousands of time and hope. Today, standard embryo rescue is done for the cross since it gives a higher % success.
Do I understand this right? Your talking about growing the new plant before an actual seed even forms? So first you have to know that a pollination has occurred but the seed either won't mature or will be deficient in some way if it does?
Nothing ruins a neighborhood like paved roads and water lines.
Post by philagardener on Dec 27, 2017 17:56:58 GMT -5
Yes, essentially growing out the young hybrid embryo to get around genetic incompatibilities that would interfere with seed development on the mother plant.
You wouldn't use the technique for crosses that can mature seed (easier to let the plant do it!); there are often clues that fertilization has taken place (like when flower doesn't immediately drop like a failed pollination, or ovary tissue that starts to swell) that suggest when the effort is worth trying.
Embryo rescue is typically only done after the natural seed formation fails. Then one watches and tries to find out when it fails. Then one decides whether one really wants that hybrid that bad, 'cause it really is extra trouble. But often it is worth the trouble. With all the history of embryo rescue, one doesn't usually have to do testcrosses to know if embryo rescue is necessary, and if so, how. That is what google is for.
keen101 (Biolumo / Andrew B.): Looking for Goldini Zucchini again. Thinking of setting up my own seed shop for OSSI varieties in the future.
Apr 2, 2022 3:58:57 GMT -5
gratefulseedsaver: I have Goldini seeds. email@example.com
Oct 8, 2022 18:46:12 GMT -5
wilscase: Hello all. My name is Casey Wilson. I'
Oct 18, 2022 21:31:32 GMT -5
wilscase: I'm a graduate student at Oregon State and have been working with populations segrgating for different color genes such as the B gene in Cucurbita. I'm curious if anyone has experience with crosses in Cucurbita maxima between grey blue types and orange?
Oct 18, 2022 21:33:14 GMT -5
wilscase: I have been backcrossing to the grey parent for 4 generations and have finally selfed the heterozygotes (for the Bmax gene) the populations have segregated for diffuse bicolor (pink/blue, orange green), blue green, blue, green, pink (salmon) and orange
Oct 18, 2022 21:36:29 GMT -5
wilscase: The genes involved are Bmax and bl. I have observed that Bmax is incompletely dominant to wild type (green). I have read that bl is incompletely recessive to Bl(wild type). I'm curious if anyone else has observed the behavior of Bmax in a grey/blue type
Oct 18, 2022 21:38:28 GMT -5
wilscase: It appears that bl and Bmax are interacting to produce different shades of salmon and pink.
Oct 18, 2022 21:38:52 GMT -5
wilscase: I'm also interested in any other color genetics, especially the relationships between B and L genes. In the right background these genes can dramatically increase Carotenoids (vitamin A)
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wilscase: I have lots of germplasm and would love to exchange anything that people are interested in
Oct 18, 2022 21:41:56 GMT -5