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Post by MikeH on Dec 31, 2013 13:00:02 GMT -5
BTW, you don't need a huge space for tissue culture unless you want to raise large numbers of explants. My current set up is a 60 gallon aquarium turned upside down, with two holes cut for glove ports. I do all work in the aquarium, then transfer the cultures to a set of shelves with shop lights at each level, boxed in with rigid foam insulation and a small space heater at the bottom. I have some other stuff that takes up more space, but those two pieces are all that is really essential. The aquarium can be put away, so the 4'wx2'dx6'h shelf is really the only permanent space given up. Even that small setup gives me a capacity for about 160 plantlets. Bill, Could you explain your setup a bit more? How difficult is to tissue culture say a woody perennial? There's a lot of DIY stuff on the net but, as usual, it's difficult to separate those who do from those who edit. Regards, Mike |
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Post by oxbowfarm on Dec 31, 2013 20:51:49 GMT -5
Hey Mike, I've got this book, Plants from Test Tubes that I find to be a pretty thorough introduction to micro-propagation. I don't know that its enough to jump in all by itself, but its enough to be able to watch some of the other stuff on the net more knowledgeably. |
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Post by billw on Dec 31, 2013 20:59:37 GMT -5
Hey Mike,
I've seen this and I'll come back to it. As it happens, I am cleaning up a document on this very subject for another member, so I'll post it here when I'm done.
The short answer for woody perennials is: it depends. Depends on the species - some are easier than others. It also depends on your goal. They can be difficult to devirus, but generally not that difficult to culture.
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Post by MikeH on Jan 1, 2014 9:54:25 GMT -5
Hey Mike, I've seen this and I'll come back to it. As it happens, I am cleaning up a document on this very subject for another member, so I'll post it here when I'm done. The short answer for woody perennials is: it depends. Depends on the species - some are easier than others. It also depends on your goal. They can be difficult to devirus, but generally not that difficult to culture. Without trying to find out by trying, is there a list of species that are easy or easy-ish? The goal is small. There are some plants that are difficult to propagate by the usual methods . I'm also not looking for large quantities. Your 160 plantlets is probably 130 more than I'd be looking at. |
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Post by ottawagardener on Jan 24, 2014 11:10:19 GMT -5
Just started to look into tissue culture so following.
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