Advanced Damaged Carrot Regeneration!

[keen101 (Biolumo / Andrew B.)]

So i am starting this thread in the hopes that you smart people can help me with this. I find this forum constantly generates great ideas on things.

So here is the problem: i have several carrots that were the main staples of my new carrot breeding program. Basically eliminated out the puny ones that flowered the first year. Then saved an emphasis on the huge ones. I had at least one giant yellow carrot and one giant purple carrot. BUT the deer ate the tops off!! Am i wrong in thinking the tops of these carrots will not regenerate from the giant taproot or will they?? Perhaps with some giberellin application? Unless someone says they will/can i will assume no.

But i am aware vaguely of another possible technique. Creating / culturing of carrot callus. I first noticed this on Carolina Biological website. (https://www.carolina.com/plant-tissue-cultures/carrot-callus-daucus-carota-plant-tissue-culture/191875.pr). But i must admit i don't really fully understand it or know much about it.

Based on this slideshow it seems as if you can create your own carrot callus cultures by taking a cross section of a carrot taproot and taking out the middle cells and sterilizing them and treating them with acid??

www.slideshare.net/JayEdneilOlivar/carrot-callus-formation

www.researchgate.net/figure/Indirect-shoot-organogenesis-in-Dieffenbachia-A-callus-formation-from-stem-segments-B_fig1_258112899

www.youtube.com/watch?v=J0sdmced1dA

link.springer.com/article/10.1023/A:1009681725540

So can someone help?

[shmack1]

If there is some of the crown left, then they will come back for sure. But if it's gone I'm not sure,but I would think even then with some tlc you might get some regrowth. Carrots are pretty tough..

[billw]

Low doses of 2,4-d have been used to get carrot callus to form adventitious shoots. That might work on the root. The hard part would be delivering the correct amount as a surface treatment. Cutting the root into discs might work better.

[keen101 (Biolumo / Andrew B.)]

Mar 17, 2018 20:25:22 GMT -5 billw said:

Low doses of 2,4-d have been used to get carrot callus to form adventitious shoots. That might work on the root. The hard part would be delivering the correct amount as a surface treatment. Cutting the root into discs might work better.

okay. interesting. i noticed that mentioned in one of the papers i linked to. I also noticed in the slideshow i linked to they had carrot slices or rings in petri dishes growing new tops somehow.

Apparently 2,4-d is a common weed killer that causes weeds to well, grow like weeds. haha. interesting. Looks easily obtainable.

Yes, how would i dilute it enough to be effective?

[steev]

Dilute it enough to get it out of the environment.