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Post by keen101 (Biolumo / Andrew B.) on Dec 30, 2017 2:17:55 GMT -5
starbuckwhy and darrenabbey - I came across a publication by the FAO/ICARDA/ICRISAT which stated they have a free gamma mutation breeding service available. "Any plant breeder can send seeds for gamma ray irradiation to this address. The scientists working there can advise on the dose of gamma rays or apply the dose requested by the breeder. They will also adjust seed moisture content if necessary." in Plant Breeding and Farmer Participation - Methodologies for generating variability. Part 4: Mutation techniques p163 www.fao.org/docrep/012/i1070e/i1070e00.htmFAO/IAEA Agriculture Laboratory, Plant Breeding Unit, A-2444 Seibersdorf, Austria (<Official.Mail@iaea. org>) Hope that helps guys. Cheers Steve I would like to learn more about this free gamma mutation breeding service. I am thinking about starting a cilantro breeding project to rid cilantro of the horrible soap tasting genes. Since no one i know of hates the taste of cilantro more than me and so many restaurants insist on putting this vile weed into EVERYTHING it makes sense that no one is better to start such a project than i. The only thing is i would want lots of cilanto seeds with as many mutations as possible to help screen for any strains that might be palatable. P.s. does anyone know of any heirloom strains of Cilantro (or the seed used as an herb Coriander which does not taste terrible like the plant)? I doubt it, but it never hurts to ask. I might be interested in other radiated plants in the near future as well. |
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Post by steev on Dec 30, 2017 2:59:00 GMT -5
Sorry you have such a distaste for cilantro, which I love; I hope you succeed in your search for an "un-cilantro" cilantro, for your own purposes, though I don't know what the benefit would really be. Have you tried parsley?
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Post by keen101 (Biolumo / Andrew B.) on Dec 30, 2017 4:59:16 GMT -5
Sorry you have such a distaste for cilantro, which I love; I hope you succeed in your search for an "un-cilantro" cilantro, for your own purposes, though I don't know what the benefit would really be. Have you tried parsley? Thanks Steev. Yes, i like parsley. But restaurants love to use cilantro. Supposedly people think cilantro tastes "fresh", a taste i can not taste as my tongue can only detect pungent and strong chemicals that taste like soap, are reminiscent of stinkbugs, and seem to make my tongue go numb. Looking on Wikipedia seems to show that the same chemicals in cilantro are actually used in soap making and are found in stinkbugs. So lol. I would LOVE for all the restaurants around me to use parsley instead of cilantro but i fear no one would agree to that. Hence my idea for such a project. If i could breed or select a cilantro strain to still taste "fresh" to the people who like cilantro while breeding or selecting out the traits that code for the soap/stinkbug chemicals then i could promote such a variety to these companies in hopes that they would adopt it nationwide. That's my mini dream anyway. One can dream anyway, no law against that. yet... |
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Post by darrenabbey on Jan 4, 2018 18:57:24 GMT -5
I would like to learn more about this free gamma mutation breeding service. I am thinking about starting a cilantro breeding project to rid cilantro of the horrible soap tasting genes. Since no one i know of hates the taste of cilantro more than me and so many restaurants insist on putting this vile weed into EVERYTHING it makes sense that no one is better to start such a project than i. The only thing is i would want lots of cilanto seeds with as many mutations as possible to help screen for any strains that might be palatable. P.s. does anyone know of any heirloom strains of Cilantro (or the seed used as an herb Coriander which does not taste terrible like the plant)? I doubt it, but it never hurts to ask. In general, it's easier to work on a breeding project with a plant that you actually like. I used to hate cilantro, but now I'm mostly neutral about it. The chemical(s) in cilantro that you find offensive might just be what others find tasty. I've tried to dig up some research on it, but I haven't found anything clarifying if those are the "tasty" compounds. I'd definitely interested to hear if you have an progress on this project. My UV mutation project still exists, but other tasks (house/car repair, etc.) keep taking priority. |
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Post by diane on Jan 5, 2018 14:16:23 GMT -5
It looks as though no one has actually zapped any seeds yet.
None sitting next to your teeth being x-rayed, none sent for gamma ray treatment.
What about setting your seeds in a tanning bed? Are they still operational?
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Post by keen101 (Biolumo / Andrew B.) on Jan 5, 2018 14:54:00 GMT -5
What about setting your seeds in a tanning bed? i think that is basically what darrenabbey was kindof doing with his UV exposure box project... |
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Post by darrenabbey on Jan 29, 2018 23:53:19 GMT -5
Yep. My device is fully functional. I just need to dedicate the time and space to growing out treated seeds and track their development closely enough to make the study worthwhile. That's the hard part.
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Post by keen101 (Biolumo / Andrew B.) on Feb 6, 2018 1:24:42 GMT -5
Yep. My device is fully functional. I just need to dedicate the time and space to growing out treated seeds and track their development closely enough to make the study worthwhile. That's the hard part. I bought one of these a while back with not a clear idea of what i wanted to use it for. I thought maybe for UV pcb etching. But, would it be suitable to make a similar device to yours for UV mutation seed treatment? I kinda want to buy some more. www.allelectronics.com/item/led-16uv/hi-intensity-uv-lamp-assembly/1.html |
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Post by darrenabbey on Feb 17, 2018 0:00:47 GMT -5
Yep. My device is fully functional. I just need to dedicate the time and space to growing out treated seeds and track their development closely enough to make the study worthwhile. That's the hard part. I bought one of these a while back with not a clear idea of what i wanted to use it for. I thought maybe for UV pcb etching. But, would it be suitable to make a similar device to yours for UV mutation seed treatment? I kinda want to buy some more. www.allelectronics.com/item/led-16uv/hi-intensity-uv-lamp-assembly/1.htmlFor any such device, you'll have to do a series of tests to determine what exposure time might be effective. The higher the intensity, the lower the time you'd need. Just make sure you've got the output contained, so you don't sunburn your corneas while you're playing around. (Just the other day I realized I have a UV-transilluminator (from lab surplus) that puts out -way- more UV than the device I built, It is painful to be around while on because of the intensity.) I still need to do the basic experimentation to see how UV treatment of tiny or small seeds would actually work. The basic idea is to figure out what exposure is sufficient to result in a 50% drop in germination rate vs. untreated control samples. At this level you'll have all sorts of mutations, but still plenty of viable seeds. Treating seeds that have been soaked in water to start them waking up would probably result in more viable mutations recovered, but this is again something that would need to be tested. Larger seeds would need more treatment time or they may be thick enough to be immune to the effect. (I imagine beans or squash could only be done after surgically removing one of the cotyledens from each seed.) |
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Post by nicollas on Feb 17, 2018 0:48:27 GMT -5
This would be fantastic if one could built a mutation breeding machine with cheap components and not much electronic skills !
Reading this "There are three ranges of UV radiation: short UV-C (100–280 nm), which is the most harmful for the genetic material as it is directly absorbed by the DNA, middle-range UV-B (280–315 nm) which is the main DNA damaging component of the solar light, and long wavelength UV-A (315–400 nm)."
Th UV lamp is at 400nM so can we deduce that it will not dammage enought the DNA to be an effective mutation breeding tool ?
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Post by keen101 (Biolumo / Andrew B.) on Feb 17, 2018 1:19:37 GMT -5
Yeah, i decided that despite my UV scrap device being sold as high intensity is probably at the wrong UV wavelength for seed mutation. It's basically just a glorified black light.
I have a blue-led transilluminator incidentally haha, but of no use for this project. Orange plexiglass does well at filtering out blue and UV light. But yes, you are right, UV light, like Infrared light can damage your eyes.
Still, i bet there might be other cheap components out there that might work. Just have to look for some. Super bright star UV leds probably could be purchased in the correct wavelength.
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Post by nicollas on Feb 17, 2018 6:13:51 GMT -5
I've read about Aquarium UV Sterilizer for mutation but i've not checked more
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Post by walt on Feb 17, 2018 14:13:17 GMT -5
Wal mart used to sell a UV toothe brush sterilizer. It was tuned to kill bacteria by destroying DNA. So I bought one for mutating pollen.
Haven't used it yet. Don't remember where it is. If I find it, i'll try it.
It was under $20. Ran on a battery. It looked like it would be real easy to wire it to a battery and switch outside its case so pollen could be put in while the electricity was off, then it could be turned on and off from safety.
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Post by nicollas on Feb 18, 2018 1:29:00 GMT -5
Walt: this is a smart idea ! I couldnt find any spec on the UV  |
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Post by nicollas on Feb 18, 2018 1:35:48 GMT -5
So it happens there are UV sterilizer boxes so all the work is done ! First link found, still need to find UV spec, but the desc states "Within the unit is a germicidal lamp. It is a special type of lamp which produces ultraviolet (UVC) light. This short-wave ultraviolet light disrupts DNA base pairing causing pyrimidine dimers formation and leads to the inactivation of bacteria, viruses, and protozoa"  www.massagesupplies.com/product/8057/ |
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